ISO INTERNATIONAL STANDARD 16212 Second edition 2017-06 Cosmetics Microbiology Enumeration of yeast and mould Cosmétiques - Microbiologie Dénombrement des levures et des moisissures Reference number ISO 16212:2017(E) International Organization for Standardization =ZHEJIANG INST OF STANDARDIZATION C1 5956617 @IS02017 vided by IHS under I No reproduction or networking permitted without license from IHS IS0 16212:2017(E) COPYRIGHT PROTECTED DOCUMENT IS0 2017, Published in Switzerland All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form written permission. Permission can be requested from either ISO at the address below or ISO's member body in the country of the requester. ISOcopyrightoffice Ch. de Blandonnet 8 · CP 401 CH-1214 Vernier, Geneva, Switzerland Tel. +41 22 749 01 11 Fax +41 22 749 09 47 [email protected] www.iso.org Internationai ibrg DrganizationforStandardization icensee-ZHEJIANG INSTOF STANDARDIZAoISQ 07 - All rights reserved Not for Resale, 2017/9/12 03:13:37 networking permited withoutlicense from IHS IS0 16212:2017(E) Contents Page Foreword ..iv 1 Scope 1 2 Normative references ..1 3 Terms and definitions 4 Principles .2 4.1 General .2 Plate count T 4.2 4.3 Membrane filtration .2 5 Diluents, neutralizers and culture media .3 5.1 General ..3 5.2 Neutralizing diluents and diluents .3 5.3 Diluent for yeast suspension (tryptone sodium chloride solution) .4 5.4 Culture media. .4 6 Apparatus and glassware 7 Strain of microorganisms ..5 8 Handling of cosmetic products and laboratory samples .6 9 Procedure ..6 9.1 General recommendation 9.2 Preparation of the initial suspension .6 9.2.1 General .6 9.2.2 Water-miscible products. .6 9.2.3 Water-immiscible products ..6 9.3 Counting methods. .6 9.3.1 Dilutions for counting methods ..6 9.3.2 Plate-count methods. .7 10 Counting of colonies (plate counts and membrane filtration methods) .7 11 Expression of results .8 11.1 Method of calculation for plate count. ..8 11.2 Interpretation, 6: 12 Neutralization of the antifungicidal properties of the product ..10 12.1 General ..10 12.2 Preparation of inoculum .11 12.3 Suitability of counting methods .11 12.3.1 Principle. .11 12.3.2 Suitability test of the pour-plate method 11 12.3.3 Suitability of the surface spread method .11 12.3.4 Suitability of the membrane filtration method .11 13 Test report. ..12 Annex A (informative) Other neutralizing diluents .13 Annex B (informative) Other diluents. ..15 Annex C (informative) Other culture media ..16 Annex D (informative) Neutralizers of antifungicidal activity of preservatives and rinsing liquids ..18 Bibliography .19 iii icensee=ZHEJIANG INST OF STANDARDIZATION C1 5956617 d without license from IHS Not for Resale, 2017/9/12 03:13:37