ISO INTERNATIONAL STANDARD 11292 First edition 1995-06-15 Corrected and reprinted 1997-02-01 Instant coffee - Determination of free and total carbohydrate contents Method using high-performance anion-exchange chromatography Café soluble - Détermination des teneurs en hydrates de carbone libres et totaux - Methode par chromatographie d'échange d'anions a haute performance OSI Reference number ISO11292:1995(E) No reproduction or networking permitted without license from IHS Not for Resale ISO 11292:1995(E) Foreword ISO (the International Organization for Standardization) is a woridwide federationofnationalstandardsbodies(lSOmemberbodies).Thework of preparing International Standards is normally carried out through iso which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with IsO, also take part in the work. IsO collaborates closely with the International Electrotechnicai Commission (lEC)onallmattersofelectrotechnicalstandardization. Draft International Standards adopted by the technical committees are a vote. ISO/TC 34, Agricultural food products, Subcommittee SC 15, Coffee. Annexes A and B of this International Standard are for information only ISO1995 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from the publisher International Organization for Standardization Case Postale 56 · CH-1211 Geneve 20 · Switzerland Printed in Switzerland CopyrightIntenational Organization for Standardization No reproduction or networking permited without license from IHS Not for Resale INTERNATIONAL STANDARD ISO ISO11292:1995(E) Instant coffee - Determination of free and total carbohydrate contents -- Method using high-performance anion-exchange chromatography 1 Scope 3Definitions This International Standard specifies a method for the For the purposes of this International Standard, the determination of free and total carbohydrate contents definitions given in IS0 3509 and the following defi- in instant coffee using high-performance anion- nitions apply. exchange chromatography. In particular, it determines the content of individual monosaccharides, sucrose 3.1 free carbohydrate content: Content of each and mannitol individual monosaccharide(arabinose,fructose galactose, glucose, mannose), and the sucrose and mannitol contents, determined under the conditions described (method A). Content is expressed as a per- 2 Normative references centage by mass on a dry basis. 3.2total carbohydrate content:Content of each The following standards contain provisions which. individual monosaccharide (arabinose, galactose through reference in this text, constitute provisions glucose, mannose, xylose) and the mannitol content, of this International Standard. At the time of publica- determined under the conditions described, which in- tion, the editions indicated were valid. All standards cludes a strong hydrolysis step (method B). Content are subject to revision, and parties to agreements is expressed as a percentage by mass on a dry basis. based on this International Standard are encouraged to investigate the possibilityof applyingthe most re- cent editions of the standards indicated below 4Principle Members of IEC and ISO maintain registers of cur rently valid International Standards. 4.1 Method A IsO 1042:1983, Laboratory glassware - One-mark Dissolution of a test portion in water. Separation of volumetric flasks. the carbohydrates present in the filtered extract by ion chromatography on a high-performance anion- ISO 3509:1989, Coffee and its products Vocabulary. eluent. Electrochemical detection of the eluted com- poundsbymeans ofapulsedamperometricdetector ISO 3726:1983,Instant coffee Determination of (PAD) and quantification by comparison with peak lossinma